Primers for Wells, J.W., Graveel, C.R., Bartley, S.M., Madore, S.J., and Farnham, P.J.

Identification of E2F1-Specific Target Genes. PNAS, 2002.

 

RT-PCR Primers

 

Gene Name 5' Primer Sequence 3' Primer Sequence
dhfr 5'-CCAGCATATGCACAGGGTAC-3' 5'-CTCTCGTCTCCATGGAACAC-3'
thymidine kinase 5'-CAGCATCTTGAACCTGGTGC-3' 5'-CTGAGAGGCAAAGAGCTTCC-3'
cyclin E 5'-GCAGAAGGTCTCAGGTTATC-3' 5'-GTGGCCTCCTTAACTTCAAG-3'
Rb 5'-TGACACAACCCAGCAGTGCG-3' 5'-CTATCCGAGCGCTCCTGTTC-3'

 

ChiPS Primers

 

Gene Name 5' Primer Sequence 3' Primer Sequence
cdc2 5'-GGTAAAGCTCCCGGGATCCGCCAAT-3' 5'-GTGGACTGTCACTTTGGTGGCTGGC-3'
PAI-1 5'-CAGACCAAGGCTCGAGGAAG-3' 5'-ATCCAGCTGTGCTCCGTTCC-3'
hydroxysteroid sulfotransferase 5'-GATTCCAGCCGAGGACACCTCT-3' 5'-AAAGGTTCTGTATGATCTCTGTCAT3'
thioether S-methyltransferase 5'-AGCCCACACAGCTGTCCAGA-3' 5'-CCTGATCCCAGTTGTCCACA-3'
carboxylesterase 5'-AGAGACAGGGAGGTGTCTGC-3' 5'-CCCAGCAGAGAAACACTGGG-3'
cylcin E 5'-AAGAACACGCCCCCCGGGAGGCCAC-3' 5'-AAGCTGTGTCCGCCGCAGGCAGGCG-3'
dhfr 5'-CGGCAATCCTAGCGTGAAGGCTGGT-3' 5'-GGCTCCATTCAGCGACGAAAGGTGC-3'
thymidine kinase 5'-CCCTAAGCCCCTTAGTTCCTGCTGG-3' 5'-CCTTAGTTAGCACCGAGTCCGTGGG-3'
Rb 5'-GAAAACCGGACGCGCCCGGCAA-3' 5'-CGTTCTCCCAGAGGCCGCGGCT-3'

 

Excel File