Primers for Eberhardy, S.E. and Farnham, P.J., 2002

Primers used for cloning Myc fusion constructs

GST-Myc Primers

Primer Name Sequence
BH Myc 1 5’-CATGCGATCGGATCCATGCCCCTCAACGTTAGC-3’
BH Myc 36 5’-CATGCGATCGGATCCCAGCAGAGCGAGCTGCAG-3’
BH Myc 41 5’-CATGCGATCGGATCCTCACTGCAGCTCGCTCTGCTGCT-3’
BH Myc 70 5’-CATGCGATCGGATCCTCAGCAGAGCCCGGAGCGGCGGCT-3’
BH Myc 103 5’-CATGCGATCGGATCCTCAGGTCACCATCTCCAGCTGGTC-3’
BH Myc 120 5’-CATGCGATCGGATCCTCACGGGTCGCAGATGAAACTC-3’
BH Myc 143 5’-CATGCGATCGGATCCTCAGGCGGCGGCCGAGAAGCCGCT-3’

 

Gal4-Myc primers

Primer Name Sequence
Sc Myc 1 5’-CATGCGATCGAGCTCATGCCCCTCAACGTTAGC-3’
Sc Myc 36 5’-CATGCGATCGAGCTCCAGCAGAGCGAGCTGCAG-3’
Sc Myc 70 5’-CATGCGATCGAGCTCTCAGCAGAGCCCGGAGCGGCGGCT-3’
Sc Myc 120 5’- CATGCGATCGAGCTCTCACGGGTCGCAGATGAAACTC-3’

 

Bold sequences indicate restriction enzyme recognition sites
BH Myc 1, BH Myc 36, Sc Myc 1 and Sc Myc 36 are 5’ primers; the rest are 3’ primers